Composite

Part:BBa_I728979:Design

Designed by: Bernice Huang   Group: iGEM07_MIT   (2007-10-23)


I14032+B0034+BBa_I728000+B0014


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 148
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After obtaining extra unexpected bands on a western blot test of bacteria transformed with Bba_I1728978, we decided to add a double terminator to see if there was leaky transcription into the vector backbone.


Source

Bba_I728978

References